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Reconstituting a lyophilized research peptide means dissolving the powder in bacteriostatic water so a precise volume can be drawn for research. Here is the standard laboratory procedure, plus a calculator to work out concentration and draw volume.

What you need

  • Your lyophilized peptide vial
  • Bacteriostatic water (0.9% benzyl alcohol)
  • A sterile syringe
  • Alcohol swabs

Step by step

  1. Swab both vial stoppers with alcohol and let dry.
  2. Draw your calculated volume of bacteriostatic water into the syringe.
  3. Inject the water slowly, letting it run down the inside wall of the vial — never spray it directly onto the powder.
  4. Swirl gently to dissolve. Do not shake — agitation can damage the peptide.
  5. Once clear, store refrigerated and protected from light.

Reconstitution calculator

Enter your vial amount, water added, and target research dose to get concentration and draw volume:

For laboratory research calculations only. Not dosing guidance for human or animal use.

For the standalone tool, see the peptide reconstitution calculator.

Shop bacteriostatic water

Research Use Only. All compounds are sold strictly for laboratory research and are not for human or animal consumption, nor for therapeutic, diagnostic, or cosmetic use.

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